In vitro Explant Cultures to Interrogate Signaling Pathways that Regulate Mouse Lung Development

In vitro Explant Cultures to Interrogate Signaling Pathways that Regulate Mouse Lung Development

Blog Article

Early mouse lung development, including specification of primordia, patterning Collections of early endoderm and determination of regional progenitor cell fates, is tightly regulated.The ability to culture explanted embryonic lung tissue provides a tractable model to study cellular interactions and paracrine factors that regulate these processes.We provide up-to-date protocols for the establishment of this culture model and its ORG DARK CHOC 70% application to investigate hedgehog signaling in the developing lung.